Turbo3C (HRV3C) Protease, Recombinant | 9206

’s Human rhinovirus 3C protease (HRV3C Protease) is a cysteine protease that recognizes the cleavage site of Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro, commonly referred to as the PreScission Site. It cleaves between Gln and Gly (independent of Pro). The recombinant form of the HRV3C protease is a restriction grade protease that has robust activity at 4°C with high specific activity and great stability. It does not require any special buffer for its activity and can be used in a buffer most suitable for the target protein. This HRV3C Protease is a 47 kDa protein with both GST and His tags so it can be easily removed by either Ni-chelating or Glutathione (GSH) resin along with the cleaved tag.

9206 | Turbo3C HRV3C Protease Recombinant DataSheet

Biomolecule/Target: N/A

Synonyms: HRV3C Protease

Alternates names: Protease

Taglines: A restriction grade protease which cleaves at the PreScission Site

NCBI Gene ID #: 7173

NCBI Gene Symbol: TPO

Gene Source: N/A

Accession #: P07202

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: The ED as determined by the dose-dependent stimulation of the proliferation of human MO7e cells was found <1 ng/ml, corresponding to a specific activity of >1 x 10 IU/mg.

Results: N/A

Binding Capacity: N/A

Unit Definition: > 1 unit/µg. One unit of Turbo3C (HRV3C) Protease cleaves >95% of 100 µg of target protein at 4°C for 16 hours

Molecular Weight:

Concentration: N/A

Appearance: Liquid

Physical form description: 2 mg/ml in 25 mM Tris-HCl, pH 8.0, 50 mM NaCl, 1 mM TCEP, and 50% glycerol

Reconstitution Instructions: Reconstitution in purified HO to a concentration of 0.1-1.0 mg/ml. The solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use. For long term storage it is recommended to add a carrier protein (0.1% HAS or BSA).

Amino acid sequence: N/A