UbcH1, human recombinant (GST-tag) (New size) | 4846

Among ubiquitin-conjugating enzymes, the mammalian ubiquitin conjugating enzyme UbcH1, is unique in its ability to catalyze the in vitro synthesis of unanchored Lys48-linked poly-ubiquitin chains from mono- or poly-ubiquitin, E1, and ATP. In addition, UbcH1 can catalyze the cyclization of longer poly-ubiquitin chains, including tetra- and penta-ubiquitin. Recombinant UbcH1 charges and supports ubiquitinylation in vitro. Typical enzyme concentration to support conjugation in vitro is 100 nM to 1 µM.

4846 | UbcH1 human recombinant GST-tag DataSheet

Biomolecule/Target: N/A

Synonyms: UbcH1, Ubiquitin conjugating enzyme 1, Ubiquitin conjugating enzyme

Alternates names: UbcH1, Ubiquitin conjugating enzyme 1, Ubiquitin conjugating enzyme

Taglines: An essential ubiquitin-conjugating enzyme

NCBI Gene ID #: 7173

NCBI Gene Symbol: TPO

Gene Source: Human

Accession #: P07202

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: The ED as determined by the dose-dependent stimulation of the proliferation of human MO7e cells was found <1 ng/ml, corresponding to a specific activity of >1 x 10 IU/mg.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight:

Concentration: 1 mg/ml

Appearance: Liquid

Physical form description: Supplied at a concentration of 1.0 mg/ml in 20 mM TBS, pH 7.5 containing 1.0 mM DTT.

Reconstitution Instructions: Reconstitution in purified HO to a concentration of 0.1-1.0 mg/ml. The solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use. For long term storage it is recommended to add a carrier protein (0.1% HAS or BSA).

Amino acid sequence: N/A