Ultra-Sensitive TMB Substrate is a one bottle stabilized chromogenic substrate for use with horseradish peroxidase (HRP) immunoassays. It contains both 3,3’,5,5’tetramethylbenzidine (TMB) and hydrogen peroxide (H₂O₂) in a one bottle format with long term stability and high sensitivity. It develops a deep blue color in the presence of HRP. The color intensity is proportional to the amount of HRP activity, which in turn is related to the levels of target analyte in an optimized ELISA, Western blotting and immunohistochemistry procedure. Addition of sulfuric acid stop solution (TBS5030) changes the color to yellow, enabling accurate measurement of the intensity at 450 nm using a spectrophotometer or plate reader. This formulation is used in most of the Tribioscience ELISA Kits where it provides consistent performance for the ELISA technique. It is ready-to-use formulation with NO DMF or DMSO.

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It is highly stable for a minimum of 36 months when stored at 4℃.