Universal Blocking Buffer is a highly effective blocking reagent capable of reducing nonspecific background staining associated with immunodetection techniques such as immunocytochemistry, ELISA, and Western blotting.

The solution is ready to use.

Applications:

Antibodies and other proteinaceous reagents tend to bind non-specifically to membranes, plates, and tissues. To minimize this nonspecific adsorption, a blocking buffer is used. Primary requirements of an ideal blocking buffer include enhancement of the signal-to-noise ratio and no cross-reactivity with the detection components. TBS’s Universal Blocking Buffer shows no cross-reactivity, as it does not contain biotin or enzymes found in non-fat milk preparations, and it blocks more effectively than serum albumins. For example, ELISA plates can be blocked in a few minutes instead of an hour. The blocking potency of the Universal Blocking Buffer is superior to most other conventional formulations in producing a high signal-to-noise ratio. Universal Blocking Buffer may also be used as an effective diluent for antibodies.

Storage :
Supplied as a working solution, size 100 mL.
Store at 4℃