Active Cathepsin B, human recombinant | 7580

Cathepsin B is a lysosomal cysteine protease. It is implicated in playing a role in protein degradation, arthritis, apoptosis, and cancer.

7580 | Active Cathepsin B human recombinant DataSheet

Biomolecule/Target: Cathepsin B

Synonyms: CTSB, CPSB

Alternates names: CTSB, CPSB

Taglines: A lysosomal protease playing a role in protein degradation, arthritis, apoptosis, and cancer.

NCBI Gene ID #: 8744

NCBI Gene Symbol: TNFL9

Gene Source: Human

Accession #: P41273

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): >7500 mU/mg (1U = 1 µmole/min digestion of Ac-Arg-Arg-AFC substrate (K140-100)).

Biological activity: Determined by the dose-dependent stimulation of IL-8 production by human PBMC. The ED for the effect is less than 10 ng/ml. Please note: results may vary with PBMC donors.

Results: >7500 mU/mg

Binding Capacity: N/A

Unit Definition: 1U = 1 µmole/min digestion of Ac-Arg-Arg-AFC substrate (K140-100).

Molecular Weight: 19.5 kDa

Concentration: 0.5 mg/ml

Appearance: Liquid

Physical form description: 0.5 mg/ml solution of protein in 25 mM sodium acetate, 50 mM NaCl, pH 5.0 containing 50% glycerol.

Reconstitution Instructions: We recommend a quick spin followed by reconstitution in HO to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.

Amino acid sequence: N/A