Active Cathepsin D, Human Recombinant | 9229

Cathepsin D is a lysosomal aspartyl protease composed of a protein dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. It is an estrogen-regulated protein associated with tissue breakdown. Levels of cathepsin D have been positively correlated with recurring breast cancers of both node negative and node positive types. Additionally cathepsin D has been associated with amyloid formation in Alzheimer’s plaques. Cathepsin D is produced initially as a pre-pro-enzyme which gets transported to lysosomes via endosomes in most cell types. In endosomes, it gets proteolyzed by unidentified proteases by removal of the pro-peptide to generate active single-chain Cathepsin D; while in lysosomes, further processing by cysteine cathepsins B and L generates mature, active double-chain Cathepsin D. ’s Active Human Cathepsin D is a proteolytically active, non-glycosylated enzyme expressed, purified and activated by proprietary methods.

9229 | Active Cathepsin D Human Recombinant DataSheet

Biomolecule/Target: Cathepsin D

Synonyms: Procathepsin D, CTSD

Alternates names: Procathepsin D, CTSD

Taglines: An active Lysosomal aspartyl protease

NCBI Gene ID #: 8744

NCBI Gene Symbol: TNFL9

Gene Source: Human

Accession #: P41273

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: 80%

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): Measured by Cathepsin D Activity Fluorometric Assay Kit and Pepstatin A inhibition using Cathepsin D inhibitor screening kit

Biological activity: Determined by the dose-dependent stimulation of IL-8 production by human PBMC. The ED for the effect is less than 10 ng/ml. Please note: results may vary with PBMC donors.

Results: BV's Cathepsin D has specific activity >100 pmol/min/mg and IC50 = 0.32 pM

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 19.5 kDa

Concentration: N/A

Appearance: Freeze-Dried

Physical form description: Freeze-dried from proprietary buffer

Reconstitution Instructions: We recommend a quick spin followed by reconstitution in HO to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.

Amino acid sequence: N/A