Active Cathepsin S, human recombinant | 7526

Cathepsin S (CTSS) is a lysosomal cysteine protease of the papain family and may participate in the degradation of antigenic proteins to peptides for presentation on MHC class II molecules. CTSS is synthesized as inactive precursor of 331 amino acids consisting of a 15-aa signal peptide, a propeptide of 99 aa, and a mature polypeptide of 217 aa. It is activated in the lysosomes by a proteolytic cleavage of the propeptide. The deduced amino acid sequence contains only one potential N-glycosylation site located in the propeptide. Compared with the abundant cathepsins B, L and H, cathepsin S shows a restricted tissue distribution, with highest levels in spleen, heart, and lung. In addition, evidences indicate that cathepsin S generates amyloid beta-peptide from amyloidogenic fragments of amyloid precursor protein (APP) in the endosomal/lysosomal compartment, and is implicated in the pathogenesis of Alzheimer’s disease and Down Syndrome.

7526 | Active Cathepsin S, human recombinant DataSheet

Biomolecule/Target :Cathepsin S

Synonyms: CTSS

Alternates names: CTSS

Taglines: A lysosomal cysteine protease participating in the degradation of antigenic proteins to peptides

NCBI Gene ID #: 1520

NCBI Gene Symbol: CTSS

Gene Source: Human

Accession #: P25774

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGE: ≥90%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: ’s Active Cathepsin S has been prepared by auto-catalytic activation of Procathepsin S (Cat # 7525) at low pH. The enzyme is fully active as seen from its ability to cleave a fluorogenic substrate Z-VVR-AFC (K144-100).

Results: >2000 mU/mg (1 U = Digestion of 1 umole/min) of Ac-VVR-AFC substrate (K144-100).

Binding Capacity: N/A

Unit Definition: 1 U = Digestion of 1 umole/min

Molecular Weight: 23.9 kDa (115-331 aa)

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from proprietary buffer.

Reconstitution Instructions: Reconstitute in 50 mM sodium acetate, 100 mM NaCl (pH 5.5) to 0.1-1 mg/ml

Amino acid sequence: N/A

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans