Active HIV-2 Protease Recombinant (GST-tagged) | 7851

HIV-2 Protease, an aspartyl protease (retropepsin), is essential for the life-cycle of HIV-2 sub-type virus. It is expressed in the infected cells as a part of Gag-Pol polyprotein from which it is auto-catalytically released after formation of an immature viral particle. The enzyme subsequently cleaves the other parts of viral polyproteins resulting in the maturation of the virus. In HIV-infected patients, the enzyme is subjected to intensive mutagenesis and mutants resistant to applied medicines are produced as a result of the selection pressure. The mutation of HIV protease's active site or inhibition of its activity disrupts HIV’s ability to replicate and infect additional cells. HIV-2 has been found to be less pathogenic than HIV-1. The mechanism of HIV-2 is not clearly defined, nor the difference from HIV-1, however the transmission rate is much lower in HIV-2 than HIV-1.

7851 | Active HIV-2 Protease Recombinant GST-tagged DataSheet

Biomolecule/Target: HIV-2 Protease

Synonyms: HIV-2 retropepsin, HIV-2 Protease (PR2), cd05482

Alternates names: HIV-2 retropepsin, HIV-2 Protease (PR2), cd05482

Taglines: 85% Pure Active Recombinant HIV2 Protease (GST tagged) for inhibitor screening assays, activity studies, selectivity profiling, western blotting, ELISA, and numerous such applications.

NCBI Gene ID #: 1673

NCBI Gene Symbol: DEFB2

Gene Source: HIV2

Accession #: O15263

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: Determined by its ability to chemoattract immature human dendritic cells using a concentration of 10-100 ng/ml.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 4.3 kDa

Concentration: 5 mg/ml

Appearance: Liquid

Physical form description: 5 mg/ml in 50 mM Sodium acetate, 100 mM NaCl, 5 mM DTT, 5 mM EDTA, pH 5.0 containing 10% glycerol

Reconstitution Instructions: Reconstitute in ddHO to a concentration of 0.1-1.0 mg/ml. The solution can then be diluted into other aqueous buffers or store d at 4°C for 1 week or –20°C for future use.

Amino acid sequence: N/A