Active Human Caspases Group I, Active Human | K241

The caspase family consists of more than 10 members. Based on their extended substrate specificities, these enzymes were divided into three major groups: Group I (contains caspase-1,-4,-5), Group II (contains caspase-2,-3,-7), and Group III (contains caspase-6,-8,-9,-10). Evidences have suggested that Group I caspases are involved primarily in the production of inflammatory cytokines, while group II and III enzymes function in apoptosis as effectors and upstream activators, respectively. The group I caspases were expressed in E. coli and routinely tested for their ability to enzymatically cleave the substrate YVAD-pNA (for caspase-1) and WEHD-pNA (for caspase-4 & -5).

K241 | Active Human Caspases Group I Active Human DataSheet

Biomolecule/Target: N/A

Synonyms: Active Human Caspases Group I, Active Human

Alternates names: N/A

Taglines: Set of recombinant proteases involved in apoptosis

NCBI Gene ID #: CASP1- 834 CASP4- 837 CASP5 -838

NCBI Gene Symbol: CASP1 CASP4 CASP5

Gene Source: Human

Accession #: CASP1- NP_150634.1 CASP4- P49662 CASP5- P51878

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: N/A

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: N/A

Results: N/A

Binding Capacity: N/A

Unit Definition: One unit of the active recombinant caspase-1,-4,-5 is the enzyme activity that cleaves 1 nmol of the caspase substrate YVAD-pNA (for caspase-1) and WEHD-pNA (for caspase-4 & -5), respectively, per hour at 37°C in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.

Molecular Weight: N/A

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized powder

Reconstitution Instructions: Reconstitute to 1 unit/µl in PBS containing 15% glycerol.

Amino acid sequence: N/A