Active Human Caspases Set I | K230

Caspases are known to play an important role in apoptosis. Caspases exist in cells as inactive pro-enzymes and mature by proteolysis to yield large and small subunits. Active caspases are heterotetramers consisting of two large and two small subunits.

K230 | Active Human Caspases Set I DataSheet

Biomolecule/Target: N/A

Synonyms: Active Human Caspases Set I

Alternates names: N/A

Taglines: Set of recombinant proteases involved in apoptosis

NCBI Gene ID #: CASP3- 836 CASP6- 839 CASP7- 840 CASP8- 841

NCBI Gene Symbol: CASP3 CASP6 CASP7 CASP8

Gene Source: Human

Accession #: CASP3- P42574 CASP6- P55212 CASP7- P55210 CASP8- Q14790

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: N/A

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: N/A

Results: N/A

Binding Capacity: N/A

Unit Definition: One unit of the recombinant human caspase-3, -6, -7, and -8 is the enzyme activity that cleaves 1 nmol of the caspase substrate DEVD-pNA, VEID-pNA, DEVD-pNA, and IETD-pNA, respectively, per hour at 37°C in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.

Molecular Weight: N/A

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized powder

Reconstitution Instructions: Reconstitute to 0.1-1 unit per µl in PBS + 15% glycerol

Amino acid sequence: N/A