Active Human Caspases Set II | K231

Caspases are known to play an important role in apoptosis. Caspases exist in cells as inactive pro-enzymes. Most of the pro-caspases are matured by proteolysis to yield large and small subunits. Active caspases are heterotetramers, each of which consists of two large and two small subunits.

K231 | Active Human Caspases Set II DataSheet

Biomolecule/Target: N/A

Synonyms: Active Human Caspases Set II

Alternates names: N/A

Taglines: Set of recombinant proteases involved in apoptosis

NCBI Gene ID #: CASP1- 834 CASP2- 835 CASP9- 842 CASP10- 843

NCBI Gene Symbol: CASP1 CASP2 CASP9 CASP10

Gene Source: Human

Accession #: CASP1-NP_150634.1 CASP2-P42575 CASP9-P55211 CASP10-Q92851

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: N/A

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: N/A

Results: N/A

Binding Capacity: N/A

Unit Definition: One unit of the recombinant human caspase-1, -2, -9, and -10 is the enzyme activity that cleaves 1 nmol of the caspase substrate YVAD-pNA, VDVAD-pNA, LEHD-pNA, and IETD-pNA, respectively, per hour at 37°C in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.

Molecular Weight: N/A

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized powder

Reconstitution Instructions: Reconstituted to 0.1-1 unit per µl in PBS.

Amino acid sequence: N/A