Active Human Caspases Set III | K232

Highly active recombinant human caspases were expressed in E. coli and purified. Active recombinant caspases are useful in screening caspase inhibitors, studying enzyme kinetics and regulation, determining substrate specificity, and as a positive control in caspase activity assays.

K232 | Active Human Caspases Set III DataSheet

Biomolecule/Target: N/A

Synonyms: Active Human Caspases Set III

Alternates names: N/A

Taglines: Set of recombinant proteases involved in apoptosis

NCBI Gene ID #: CASP1 834 CASP2- 835 CASP3- 836 CASP6- 839 CASP7- 840 CASP8- 841 CASP9- 842 CASP10- 843

NCBI Gene Symbol: CASP1 CASP2 CASP3 CASP6 CASP7 CASP8 CASP9 CASP10

Gene Source: Human

Accession #: CASP1- NP_150634.1 CASP2- P42575 CASP3- P42574 CASP6- P55212 CASP7- P55210 CASP8- Q14790 CASP9- P55211 CASP10- Q92851

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: N/A

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: N/A

Results: N/A

Binding Capacity: N/A

Unit Definition: One unit of the recombinant human caspases is the enzyme activity that cleaves 1 nmol of the individual caspase substrate (YVAD-pNA for caspase-1, VDVAD-pNA for caspase-2, DEVD-pNA for caspase-3, VEID-pNA for caspase-6, DEVD-pNA for caspase-7, IETD-pNA for caspase-8, LEHD-pNA for caspase-9, and IETD-pNA for caspase-10) per hour at 37°C in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.

Molecular Weight: N/A

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized powder

Reconstitution Instructions: The active recombinant caspases can be reconstituted to 0.1-1 unit per µl in PBS or the Reaction Buffer described above (available from , Cat.# 1068-20) for longer stability. We recommend using 1 unit per assay for analyzing caspase activity.

Amino acid sequence: N/A