AGO4 | Argonaute 4 | AS09 617

AGO4 | Argonaute 4 | AS09 617 | Gentaur UK, US & Europe Distribution

Immunogen: KLH-conjugated synthetic peptide chosen from Arabidopsis thaliana AGO4 sequence UniProt: Q9ZVD5, TAIR: At2g27040

Host: Rabbit

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Immunogen affinity purified serum in PBS pH 7.4.

Format: Lyophilized

Tested Application: CoImmunoprecipitation (IP) (coIP), Immunocytochemistry (ICC), Immunoprecipitation (IP), Western blot (WB)

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Recommended Dilutions: 1 : 100 (ICC), 5 µg of antibody per 1 gram of a fresh tissue (IP), 1 : 2000-1 : 5000 (WB)

Molecular weight: 103 kDa

Confirmed Reactivity: Arabidopsis thaliana, Hyacinthus orientalis

Predicted Reactivity: Arabidopsis thaliana

Not reactive in: Brassica oleracea, Hordeum vulgare, Solanum lycopersicum, Vigna angularis, Zea mays

Additional Information: AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure. Use a 6 % gel for protein separation, which is run longer to avoid a cross-reactivity at ca. 40 kDa.Binds endogenous siRNAs, preference for 24nt siRNAs with 5' A.Note that the AGO4 antibody reacts with the NEB prestained protein marker.

Background: AGO4 (Argonaute 4) belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC) . This protein complex is responsible for the gene silencing (RNAi) .

Reconstitution: For reconstitution add 50 µl of sterile water

Storage: Store lyophilized/reconstituted at -20°C; Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur adhering to the cap or sides of the tube.

TAIR Nnumbre: AT2G27040

Category: microRNA

Research Area: RNAi/siRNA/miRNA