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Alkaline phosphatase, mouse recombinant | P1098
- SKU:
- 26-P1098-GEN
- Availability:
- Usually shipped in 5 working days
Description
Alpl, also known as alkaline phosphatase, tissue-nonspecific isozyme, is one of alkaline phosphatases. There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue-nonspecific). The product of this gene is a membrane-bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. A proposed function of this form of the enzyme is matrix mineralization. Recombinant mouse Alpl, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
P1098 | Alkaline phosphatase mouse recombinant DataSheet
Biomolecule/Target: Alpl
Synonyms: Alkaline phosphatase, tissue-nonspecific isozyme, Alpl, Akp-2, Akp2, ALP, APTNAP, TNAP, TNSALP, HOPS
Alternates names: Alkaline phosphatase, tissue-nonspecific isozyme, Alpl, Akp-2, Akp2, ALP, APTNAP, TNAP, TNSALP, HOPS
Taglines: A hydrolase enzyme responsible for removing phosphate groups
NCBI Gene ID #: 11647
NCBI Gene Symbol: Aplp
Gene Source: Mouse
Accession #: P09242
Recombinant: Yes
Source: Insect cell (Baculovirus)
Purity by SDS-PAGEs: 95%
Assay: SDS-PAGE
Purity: N/A
Assay #2: N/A
Endotoxin Level: < 1 EU/g
Activity (Specifications/test method): Measured by its ability to hydrolyze 1pmole of 4-Methylumbelliferyl phosphate to phosphate and 4-Methylumbelliferone per minute at pH 8.8 at 25C.
Biological activity: Specific activity is > 46,000 pmol/min/ug and is defined as the amount of enzyme that hydrolyze 1pmole of 4-Methylumbelliferyl phosphate to phosphate and 4-Methylumbelliferone per minute at pH 8.8 at 25C.
Results: > 46,000 pmol/min/ug
Binding Capacity: N/A
Unit Definition: One unit of enzyme is defined as the amount of enzyme that hydrolyze 1pmole of 4-Methylumbelliferyl phosphate to phosphate and 4-Methylumbelliferone per minute at pH 8.8 at 25C.
Molecular Weight: 54.5 kDa
Concentration: 0.5 mg/ml
Appearance: Liquid
Physical form description: In Phosphate Buffered Saline (pH 7.4) containing 10% glycerol
Reconstitution Instructions: N/A
Amino acid sequence: VPEKERDPSY WRQQAQETLK NALKLQKLNT NVAKNVIMFL GDGMGVSTVT AARILKGQLH HNTGEETRLE MDKFPFVALS KTYNTNAQVP DSAGTATAYL CGVKANEGTV GVSAATERTR CNTTQGNEVT SILRWAKDAG KSVGIVTTTR VNHATPSAAY AHSADRDWYS DNEMPPEALS QGCKDIAYQL MHNIKDIDVI MGGGRKYMYP KNRTDVEYEL DEKARGTRLD GLDLISIWKS FKPRHKHSHY VWNRTELLAL DPSRVDYLLG LFEPGDMQYE LNRNNLTDPS LSEMVEVALR ILTKNLKGFF LLVEGGRIDH GHHEGKAKQA LHEAVEMDQA IGKAGAMTSQ KDTLTVVTAD HSHVFTFGGY TPRGNSIFGL APMVSDTDKK PFTAILYGNG PGYKVVDGER ENVSMVDYAH NNYQAQSAVP LRHETHGGED VAVFAKGPMA HLLHGVHEQN YIPHVMAYAS CIGANLDHCA WAGSGLEHHH HHH