Caspases Group III, Active Human | K243

Caspase family consists of more than 10 members. Based on their extended substrate specificities, these enzymes were divided into three major groups: Group I (contains caspase-1,-4,-5), Group II (contains caspase-2,-3,-7), and Group III (contains caspase-6,-8,-9,-10). Evidences have suggested that Group I caspases are involved primarily in the production of inflammatory cytokines, while group II and III enzymes function in apoptosis as effectors and upstream activators, respectively. The Group III recombinant caspases were expressed in E. coli and routinely tested for their ability to enzymatically cleave the substrate VEID-pNA (for caspase-6), IETD-pNA (for caspase-8 & -10) and LEHD-pNA (for caspase-9), respectively.

K243 | Caspases Group III Active Human DataSheet

Biomolecule/Target: N/A

Synonyms: Caspases Group III, Active Human

Alternates names: N/A

Taglines: Set of recombinant proteases involved in apoptosis

NCBI Gene ID #: 10563

NCBI Gene Symbol: CXCL13

Gene Source: Human

Accession #: O43927

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: N/A

Results: N/A

Binding Capacity: N/A

Unit Definition: One unit of the active recombinant caspase-6,-8,-9 & -10 is the enzyme activity that cleaves 1 nmol of the caspase substrate VEID-pNA (for caspase-6), IETD-pNA (for caspase-8 & -10), and LEHD-pNA (for caspase-9), respectively, per hour at 37°C in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.

Molecular Weight: N/A

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized powder

Reconstitution Instructions: Reconstitute to 1 unit per µl in PBS containing 15% glycerol

Amino acid sequence: N/A