HSPC/CHOL/mPEG-DSPE liposomes  with ammonium gradient is the formulator’s choice in pharmaceutical/biotech industry for both formulation feasibility studies and liposomal product development, because HSPC is essentially equivalent to DSPC (HSPC contains about 85 % DSPC 15% DPPC). The main advantages for using HSPC are its lower cost and availability of GMP quality raw materials. HSPC material used at FormuMax is  from one of the world’s premier lipid vendors.

Specifications:

• Lipid composition: HSPC/CHOL/mPEG2000-DSPE (50:45:5 mol/mol)
• Mean particle size: 100 nm (95 – 120 nm)
• Lipid concentration: 60 mM(60 – 65 mM)
• Hydration buffer (loading battery): 250 mM ammonium sulfate
• External solution: 10% sucrose, 20mM sodium PO4, pH 6.5 ± 0.2

Application :

• A drug loaidng battery of 250 mM ammonium sulfate is typically used, however higher concentrations of ammonium sulfate is also ues to achieve increased drug loading capacity and drug retention.

Storage conditions :

• 2-8 °C

Shelf-life:

• 6 months