Human CellExp™ Biotinylated CD28, Human Recombinant | P1669

Human CD28 is composed of four exons encoding a protein of 220 amino acids that is expressed on the cell surface as a glycosylated, disulfide-linked homodimer of 44 kDa. Members of the CD28 family share a number of common features. These receptors consist of paired V-set immunoglobulin superfamily (IgSF) domains attached to single transmembrane domains and cytoplasmic domains that contain critical signaling motifs. The CD28 and CTLA4 ligands, CD80 and CD86, consist of single V-set and C1-set IgSF domains. The interaction of these costimulatory receptors with ligands is mediated through the MYPPPY motif within the receptor V-set domains. CD28 is expressed constitutively on almost all human CD4 T cells and approximately 50% of CD8 T cells. CD28 costimulation has diverse effects on T cell function, including biochemical events at the immunological synapse, downstream phosphorylation and other post-translational modifications, transcriptional changes, and cytoskeletal remodeling. At the most basic level, CD28 signals increase a cell’s glycolytic rate, allowing cells to generate the energy necessary for growth and proliferation.

P1669 | Human CellExp™ Biotinylated CD28, Human Recombinant DataSheet

Biomolecule/Target :

Synonyms: T-cell-specific surface glycoprotein CD28; CD antigen: TP44; Tp44; T-Cell-Specific Surface Glycoprotein; CD28 Molecule

Alternates names: T-cell-specific surface glycoprotein CD28; CD antigen: TP44; Tp44; T-Cell-Specific Surface Glycoprotein; CD28 Molecule

Taglines: Involved in T-cell activation and survival

NCBI Gene ID #: 940

NCBI Gene Symbol:

Gene Source: Human

Accession #: P10747

Recombinant: True

Source: HEK 293 cells

Purity by SDS-PAGE: > 95% by SDS-PAGE

Assay:

Purity:

Assay #2:

Endotoxin Level:

Activity (Specifications/test method): Biotinylated human CD28-Fc can bind to immobilized Human CD80, Fc Tag (Cat. No. P1668 coated at 1 ug/mL, 100 uL/well) with a linear range 0.01 – 0.27ug/ml.

Biological activity:

Results:

Binding Capacity:

Unit Definition:

Molecular Weight: ~42 kDa (Human Fc Tag fused to C-terminus, Biotinylated)

Concentration:

Appearance: Lyophilized protein

Physical form description: Lyophilized powder

Reconstitution Instructions:

Amino acid sequence: Asn 19 - Pro 152

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans