Human Recombinant Ubiquitin-K48 | 6400

Ubiquitin is a small polypeptide that can be conjugated via its C-terminus to amine groups of lysine residue on target proteins. This conjunction is referred to as monoubiquitylation. Additional ubiquitin moieties can be subsequently conjugated to this initial ubiquitin, utilizing any one of the seven lysine residues on the surface of ubiquitin. The formation of these ubiquitin chains is referred to as polyubiquitylation. This tag-free recombinant form of human ubiquitin is engineered to have all available lysines mutated to arginines, except at position 48. This molecule, therefore, can only form polyubiquitin chains of K48 linkage type. Covalent attachment of ubiquitin to other proteins serves various functions, but its major role is to target cellular proteins for destruction. Cellular components that activate, transfer, remove, or simply recognize ubiquitin number in the hundreds, perhaps even in the thousands. In light of this complexity the ubiquitin pathway is ideal for a systems biology approach. Ubiquitin plays a very important role in regulated non-lysosomal ATP dependent protein degradation. The Ub-proteasome proteolytic pathway, which is a complex process, is implicated to be of great importance for regulating numerous cellular processes.

6400 | Human Recombinant Ubiquitin-K48 DataSheet

Biomolecule/Target: N/A

Synonyms: Human Recombinant Ubiquitin-K48

Alternates names: Aldo-keto reductase family 1, member C 1, 20-alpha-HSD, DD1/DD2, HBAB, DDH, DDH1

Taglines: Serves as a substrate for K48 polyubiquitination

NCBI Gene ID #: 1645

NCBI Gene Symbol: AKR1C1

Gene Source: Human

Accession #: Q04828

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 90%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: Specific activity is approximately 0.15 – 0.2 units/mg.

Results:

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 38.9 kDa

Concentration: 4 mg/ml

Appearance: Liquid

Physical form description: 4 mg/ml in 50 mM Tris, pH 7.5, 150 mM NaCl, 10% glycerol.

Reconstitution Instructions: N/A

Amino acid sequence: MGSSHHHHHH SSGLVPRGSH MDSKYQCVKL NDGHFMPVLG FGTYAPAEVP KSKALEATKL AIEAGFRHID SAHLYNNEEQ VGLAIRSKIA DGSVKREDIF YTSKLWCNSH RPELVRPALE RSLKNLQLDY VDLYLIHFPV SVKPGEEVIP KDENGKILFD TVDLCATWEA VEKCKDAGLA KSIGVSNFNR RQLEMILNKP GLKYKPVCNQ VECHPYFNQR KLLDFCKSKD IVLVAYSALG SHREEPWVDP NSPVLLEDPV LCALAKKHKR TPALIALRYQ LQRGVVVLAK SYNEQRIRQN VQVFEFQLTS EEMKAIDGLN RNVRYLTLDI FAGPPNYPFS DEY