K11-linked Tri-Ubiquitin | 6422

Increasingly, researchers are focusing on the role poly-ubiquitin chains linked through K11. This post-translational modification has been linked to the ERAD cycle as a signal, similar to K48 linkage, for proteasomal degradation. More recently, K11 linkage appears to play an important role in cell cycle signaling, as it is associated with the anaphase promoting complex (APC) of ubiquitination machinery. These tri-ubiquitin chains are generated from the enzymatic linkage (UBE2S) of wild-type ubiquitin through lysine 11 and purified to >95% homogeneity by ion exchange chromatography.

6422 | K11-linked Tri-Ubiquitin DataSheet

Biomolecule/Target: N/A

Synonyms: K11-linked Tri-Ubiquitin

Alternates names: N/A

Taglines: Substrate for defined length polyubiquitination

NCBI Gene ID #: 14825

NCBI Gene Symbol: GROA

Gene Source: N/A

Accession #: P12850

Recombinant: N/A

Source: N/A

Purity by SDS-PAGEs: 98%

Assay: Western Blot

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: Determined by its ability to chemoattract total human neutrophils population using a concentration range of 10-100 ng/ml.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 25.656 kDa (Band migrates faster on Gels)

Concentration: 2.5 mg/ml

Appearance: Liquid

Physical form description: 2.5 mg/ml in 20 mM Tris-HCl, pH 7.5, 0.15 M NaCl and 1 mM EDTA.

Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1 mg/ml. This solution can be diluted into other aqueous buffers or stored at 4°C for 1 week or –20°C for future use.

Amino acid sequence: N/A