Sulfo-NHS-SS-Biotin | 2323

KEY FEATURES: • Sulfo-NHS-SS-Biotin is amine-reactive and • Has a long chain spacer arm (24.3 Å) separating the biotin group from the amino-reactive sulfo-NHS ester • Water-soluble; can be used under physiologic conditions • It is possible to cleave the biotin group from the cross-linked target protein under reducing conditions, such as in the presence of BME, DTT or TCEP APPLICATIONS: • Sulfo-NHS-SS-Biotin is ideal for biotinylating antibody or protein that can then be used for protein purification, detection or immobilization • Sulfo-NHS-Biotin is particularly useful for targeting cell surface proteins due to the membrane-impermeability of this reagent

Sulfo-NHS-SS-Biotin is a water-soluble, dislulfide-reducible, protein biotinylation reagent.

2323 | Sulfo-NHS-SS-Biotin DataSheet

Alternate Name/Synonyms: Sulfosuccinimidyl-2-(biotinamido)-ethyl-1,3'-dithiopropionate; Biotin disulfide N-Hydroxysulfosuccinimide ester

Appearance: White to off-white solid

Formulation: N/A

CAS Number: 325143-98-4

Structure Available?: Yes

Peptide sequence: N/A

Salt Form: No

Molecular Formula: C₁₉H₂₇O₉N₄S₄Na

Molecular Weight: 606.69

Cell-Permeable?: No

Purity: ≥80% by NMR

Solubilities: H₂O (~ 6 mg/ml)

Handling: Protect from moisture

Country of Origin: USA

Tag Line: A water-soluble, dislulfide-reducible, protein biotinylation reagent.

MDL Number: MFCD00078533

PubChem CID: N/A

SMILES: O=C(NCCSSCCC(ON1C(C(S(=O)(O[Na])=O)CC1)=O)=O)CCCCC2SCC(C2N3)NC3=O

InChi: InChI=1S/C19H30N4O8S4.Na/c24-15(4-2-1-3-13-17-12(11-32-13)21-19(27)22-17)20-7-10-34-33-9-6-16(25)31-23-8-5-14(18(23)26)35(28,29)30;/h12-14,17H,1-11H2,(H,20,24)(H2,21,22,27)(H,28,29,30);/q;+1/p-1

InChi Key: HBLXCNOBPVOLAK-UHFFFAOYSA-M