EnzyFluo™ Isocitrate Assay Kit

Application: For quantitative determination of isocitrate and evaluation of drug effects on its metabolism.

Key Features:

• Fast and sensitive. Linear detection range (20 μL sample): 0.6 to 500 μM for 10 min reaction.
• Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Method: FL530/585 nm

Samples: Food, beverage, biological samples (e.g. cell lysate, tissue homogenate, serum, etc.)

Species: All

Size: 100 tests

Detection Limit: 0.6 µM

Shelf Life: 6 months

More Details:

ISOCITRATE (ISOCITRIC ACID) is a substrate in the citric acid (TCA) cycle. Isocitrate is formed by the isomerization of citrate catalyzed by the enzyme aconitase. Isocitrate is oxidized by isocitrate dehydrogenase producing α-ketoglutarate and generating NADPH. Isocitrate is commonly found in many fruits and vegetables and their processed products. Industrially, isocitrate is used as a marker to identify the quality and purity of fruit juices.

Gentaur Genprice' isocitrate assay measures the NADPH generated from the oxidation of isocitrate. The NADPH reduces a probe into a highly fluorescent product. The fluorescence intensity of this product, measured at λex/em = 530/585 nm, is proportional to the isocitrate concentration in the sample.