RadioImmunoPrecipitation Assay (RIPA) Buffer is used to lyse cells and tissues under nondenaturing conditions.

Compoents in 1x RIPA Buffer:

• 20mM Tris-HCl (pH 7.5)
• 150mM NaCl
• 1mM Na2EDTA
• 1mM EGTA 1% NP-40
• 2.5mM sodium pyrophosphate
• 1mM β-glycerophosphate
• 1mM Na3VO4 1 µg/ml leupeptin

Directions for Use:

• Aliquotting of RIPA buffer is recommended if many small experiments are to be performed. For longer periods of time, buffer should be stored at –20°C. If buffer will be continually used, it is recommended that the buffer be kept at 4°C for 1-2 weeks.
• Thaw RIPA buffer at room temperature, mixing end-over-end.
• Chill RIPA buffer on ice and add PMSF (final 1 mM) just prior to use.

Storage Conditions:

Supplied as a ready to use solution, 50mL. 100mM PMSF, 0.5mL.
Store at –20℃. For short-term storage (1-2 weeks), RIPA Buffer can be stored at 4͓.